Pre-Conference Bootcamp

NK Cell Biology Bootcamp

Tuesday, March 23

10.30 - 4.00 EDT | 07.30 - 1.00 PDT - All times shown in EDT

10:30 am Online Registration & Virtual Coffee Networking

10:30 am Online Registration & Virtual Coffee Networking

11:00 am Super-Charged Natural Killer Cells Target and Differentiate Cancer Stem Cells/Poorly Differentiated Tumors While Expanding CD8+ T Cells for Targeting of Differentiated Tumors; Studies of Cancer Patients and Humanized Mice

11:00 am Super-Charged Natural Killer Cells Target and Differentiate Cancer Stem Cells/Poorly Differentiated Tumors While Expanding CD8+ T Cells for Targeting of Differentiated Tumors; Studies of Cancer Patients and Humanized Mice

Synopsis

• In cancer patient derived tumors, NK cells bind and interact with stem-like tumors within the tumor microenvironment
• Analyze super-charged NK cells which have significantly increased capacity to target cancer stem cells/poorly differentiated tumors in 2D and 3D tumorospheres, and drive differentiation of stem cells within tumorospheres
• Discuss how, unlike activated primary NK cells, super-charged NK cells do not become inactivated after interaction with tumors within the tumorospheres, thus mediating serial killing

11:30 am Dynamics of Vg9Vd2 T Cells Cytotoxicity

11:30 am Dynamics of Vg9Vd2 T Cells Cytotoxicity

Synopsis

• Describe expansion and phenotypic evolution of Vg9Vd2 T cells from peripheral blood
• Outline a microchip platform for live-cell imaging of thousands of individual cells within larger populations and show how we use it to assess heterogeneity in cytotoxic response and serial killing by gamma delta T cells
• Share imaging-based assessment of the cytotoxic mechanisms used by gamma delta T cells

12:00 pm Morning Refreshments

12:00 pm Morning Refreshments

12:30 pm Discussion Session: What Is the Role of Inhibitory Receptors?

12:30 pm Discussion Session: What Is the Role of Inhibitory Receptors?

1:00 pm Understanding the Biology and Molecular Program NK Cell Memory

  • Todd Fehniger Professor of Medicine, Washington University School of Medicine in St Louis

Synopsis

• Outline the critical understanding of memory and memory-like NK cells and their attributes

• Review distinct types of NK cell memory and memory-like responses

• Present the current understanding of the molecular program of memory-like NK cells

• Relate recent pre-clinical work supporting memory-like NK cells in solid tumors

1:00 pm Understanding the Biology and Molecular Program NK Cell Memory

  • Todd Fehniger Professor of Medicine, Washington University School of Medicine in St Louis

1:30 pm Single-Cell Transcriptomics of Human NK Cells

1:30 pm Single-Cell Transcriptomics of Human NK Cells

Synopsis

• Single-cell transcriptome reveals significant heterogeneity within the bone marrow-derived human NK cells, grouped into
six major unique subsets
• The functionally mature CD57+ NK cells are marked with high expression of CX3CR1, HAVCR2 (TIM 3), and ZEB2, demonstrating unique transcriptional features. Adaptive (‘memory’) NK cells express a unique transcriptomic profile along with the high expression of NKG2C
• Explain how the transcriptomic profiles NK clusters, pseudotime trajectory of developmental progression, and the identification of major regulons offer exceptional new insights essential for NK cell based immunotherapies

2:00 pm Refreshment Break

Synopsis

Time to message those you would like to connect with using the online chat function, or simply take some time away from the screen to rest your eyes!

2:00 pm Refreshment Break

2:30 pm Genetic Manipulation of Chemotactic Receptors as a Novel Strategy to Modulate the Homing of Adoptively Transferred Ex Vivo Expanded NK Cells

  • Emily Levy Postdoctoral Research Fellow, Laboratory of Transplantation Immunotherapy, NHLBI, NIH
  • Richard Childs Rear Admiral, Commissioned Corps Unites States Public Health Service, NHLBI , NIH

Synopsis

• Ex vivo expansion of NK cells with feeder cells induces transcriptional shifts in thousands of protein coding genes
• Expanded NK cells have significant alterations in chemokine receptors (CRs) including downregulation of CXCR4 and upregulation of CCR5 and other CRs which may lead to preferential NK cell trafficking to the liver rather than the bone marrow
• Genetic modification of chemotactic receptors through transfection with CXCR4 mRNA and/or CRISPR/Cas-9 disruption of CCR5 can be used to redirect the cellular homing of adoptively infused NK cells in vivo to their desired tissue target

2:30 pm Genetic Manipulation of Chemotactic Receptors as a Novel Strategy to Modulate the Homing of Adoptively Transferred Ex Vivo Expanded NK Cells

  • Emily Levy Postdoctoral Research Fellow, Laboratory of Transplantation Immunotherapy, NHLBI, NIH
  • Richard Childs Rear Admiral, Commissioned Corps Unites States Public Health Service, NHLBI , NIH

3:00 pm NK Cell Activation in the Tumor Microenvironment

Synopsis

• Overview of the NK cell – cancer immunity cycle
• Highlight emerging strategies to therapeutically modulate NK cell activity in cancer
• Drugging cancer cells to convert ‘cold’ tumours into ‘hot’ tumours

3:00 pm NK Cell Activation in the Tumor Microenvironment

3:30 pm Innate Cell Engagers (ICE®) to Trigger Innate Immunity to Fight Cancer

  • Joachim Koch Head of Translational Research & Innovation, Affimed

Synopsis

– Review pre-clinical and clinical development of Innate Cell Engagers (ICE®), derived from the Redirected Optimized Cell Killing (ROCK®) platform
– Present the current development landscape of ICE® in hematological and solid cancer
– Present recent pre-clinical in vitro and in vivo work demonstrating efficacy and novel insights into the mode-of action of ICE® triggering Antibody-Dependent Cellular Cytotoxicity (ADCC) and Antibody Dependent Cellular Phagocytosis (ADCP)
– Provide preclinical foundation and future perspective to combine ICE® and adoptive cellular NK cell therapy (“in-situ-CAR-NK”) to treat cancer patients

3:30 pm Innate Cell Engagers (ICE®) to Trigger Innate Immunity to Fight Cancer

  • Joachim Koch Head of Translational Research & Innovation, Affimed