Genetic Manipulation of Chemotactic Receptors as a Novel Strategy to Modulate the Homing of Adoptively Transferred Ex Vivo Expanded NK Cells

Time: 2:30 pm
day: Workshop


• Ex vivo expansion of NK cells with feeder cells induces transcriptional shifts in thousands of protein coding genes
• Expanded NK cells have significant alterations in chemokine receptors (CRs) including downregulation of CXCR4 and upregulation of CCR5 and other CRs which may lead to preferential NK cell trafficking to the liver rather than the bone marrow
• Genetic modification of chemotactic receptors through transfection with CXCR4 mRNA and/or CRISPR/Cas-9 disruption of CCR5 can be used to redirect the cellular homing of adoptively infused NK cells in vivo to their desired tissue target