Conference Day Two
Wednesday March 5, 2025

8:00 am Morning Coffee

Accelerating NK Therapy Development Through Clarity of Standards & Regulatory Expectations

8:30 am Panel Discussion: Defining NK Manufacturing Standards for Consistent Product Quality & Synchronized Efforts

  • Yana Wang Senior Director Cell Therapy Oncology Drug Discovery Unit, Takeda Pharmaceutical
  • Chris Horan Chief Technical Operations Officer, Artiva Biotherapeutics, Inc
  • Henri Bayle Chief Scientific Officer, NKILT Therapeutics
  • John Yu Co-Founder, Apexcella

Synopsis

  • Examine key regulatory requirements and compliance issues for NK cell manufacturing, ensuring adherence to industry standards
  • Explore strategies to synchronize manufacturing processes to maintain consistent product quality and performance
  • Discuss key performance indicators and benchmarks for evaluating NK cell manufacturing quality and process improvements

9:00 am Case Study: Key Considerations for Developing a Combination Product

Synopsis

  • Discussing the development of g-NK cell therapy with antibody combinations for NHL and AML
  • Understanding how to navigate regulations and patient treatment procedures with multiple treatment regiments
  • Highlighting positive clinical data reinforcing the rationale behind a combination approach

9:30 am Discussing Approaches when Developing an NK Product Potency Assay to Ensure Compliance

Synopsis

  • Explore how potency is defined for NK cell therapies, including the critical factors that influence their therapeutic effectiveness
  • Discuss best practices for designing robust and reproducible potency assays, including selection of appropriate biological endpoints and assay methodologies
  • Identify and discuss common challenges in developing NK product potency assays, including troubleshooting and optimizing assay performance

10:00 am Session Reserved for ScaleReady

10:30 am Morning Break & Networking

Preclinical Development

Ensuring Proper Localization of NK Cells for On- Target Efficacy

11:30 am Leveraging Image-Guided Delivery of NKs to Tumor Targets

  • Jason Chiang Professor - Interventional Radiology, University of California Los Angeles

Synopsis

  • Learn about cutting-edge approaches to circumvent NK localization hurdles by directly delivering them to tumor sites
  • Delve into radiological approaches to deliver NK therapies to the appropriate site

12:00 pm Developing CAR-NKs for Increased Accuracy of Targeting

  • Pinaki Banerjee Assistant Professor & Director - Rezvani Lab, MD Anderson Cancer Center

Synopsis

  • Discuss strategies for designing effective CAR constructs, including the selection of target antigens, costimulatory domains, and optimizing CAR expression in NK cells
  • Identify common challenges in developing CAR-NK therapies, such as CAR construct stability, NK cell expansion, and persistence

12:30 pm Chimeric ILT-Receptor (CIR™) Technology Effectively Targets HLA-G+ Hematologic & Solid Tumors for Fit-for- Purpose Activation of NK Cells

Synopsis

  • Diving on how CIR™NK cells with fit-for-purpose activation can efficiently and persistently target cancer cells across multiple tumors
  • Understanding in vivo performance and functional activity of CIR™NK cells in leukemia and solid tumors

Clinical Manufacturing & Development

Maximizing NK Expansion for a Robust & Effective Scale-Up of an Allogeneic Product

11:30 am Optimizing Yield & Function of Cytokine-Induced Memory Like (CIML) NK Cells

  • Neil Sheppard Director, Research Technologies & Innovation, Center for Cellular Immunotherapies, University of Pennsylvania

Synopsis

  • Application of feeder cell technology to expand NK cells pre or post CIML programming
  • Demonstrating feeder cell-based process leads to >700-fold greater yield of functionally superior CIML NK cells compared to published CIML method

12:00 pm Improving the Metabolic Fitness of NK Cells via the Manufacturing Process

  • Yana Wang Senior Director Cell Therapy Oncology Drug Discovery Unit, Takeda Pharmaceutical

Synopsis

  • Leveraging a feeder cell-based process to maximize potency and antitumor efficacy of expanded donor-derived NK cells
  • Understanding key aspects of the process that contribute to enhanced metabolic fitness of cells

12:30 pm Maximizing NK Cell Viability & Cytotoxicity Through Novel Expansion Technology

Synopsis

  • Leveraging the apexNK™ platform for high purity affordable NK expansion, without the need for sorting or feeder cells
  • Highlighting preliminary efficacy of NK cells expanded this way to target lymphomas and solid tumors
  • Successfully creating targeted apexNK™ cells utilizing a non-viral ACC™ chemical conjugation technology, specifically designed for blood NK cells

1:00 Lunch Break & Networking

Lengthening NK Cell Persistence for Prolonged Therapeutic Benefit

2:00 pm Developing Allogeneic Evasion to Reduce Host Attack

  • Karl-Johan Malmberg Professor, Director The Precision Immunotherapy Alliance, Institute for Cancer Research

Synopsis

  • Analyze the underlying causes of immune rejection in allogeneic NK cell therapies and their impact on treatment efficacy
  • Explore engineering NK cells with host evasion mechanisms to avoid recognition and rejection
  • Evaluate approaches to make cell surfaces “slippery” for enhanced NK persistence

2:30 pm Engineering NKs in Combination with Antibodies for Longer Lifespans & Prolonged Effect

Synopsis

  • Discussing NK combination therapy for multiple myeloma
  • Diving into NK therapy mechanisms in combination with monoclonal antibodies

Improving our Understanding of Clinical NK Performance for Best Patient Outcomes

2:00 pm Optimizing Patient Selection Through Advanced Stratification Algorithms to Ensure Clinical Benefit of NK Therapy

  • Evren Alici Co-Director - Next Generation NK Cell Competence Center & Research Group Leader, Karolinska Institutet

Synopsis

  • Discover which biomarkers are most indicative of NK therapy efficacy and patient response
  • Understand how to link specific biomarkers with clinical outcomes to better predict treatment success and patient prognosis

2:30 pm Panel Discussion: Evaluating Clinical Potential of Different NK Subtypes

Synopsis

  • Discuss how different NK subtypes perform against various cancer types and disease models
  • How do different NK phenotypes influence therapeutic outcomes?
  • Is it best to have a homogenous or heterogenous NK product?

3:00 pm Afternoon Break & Networking

Exploring the Applications of NK Biology to Tackle Infectious Diseases

3:30 pm Leveraging an IL-15 Superagonist to Activate NK Cells Against HIV Infected T-Cells

  • Jeffrey Safrit Senior Director - Infectious Diseases, ImmunityBio, Inc.

Synopsis

  • Sharing insights into mechanisms of NK cell activation to target HIV infected T-cells
  • Discussing the “kick and kill” strategy when deploying a super agonist to activate dormant HIV infected cells to target them for elimination

4:00 pm Developing a Functional Cure for Hep B HIV Infection via NK Cells & its Applications to Hepatocellular Carcinoma

Synopsis

  • Harnessing an optimized platform for expansion of adaptive memory like NK cells and demonstrating how this platform can be modified to develop immunosuppressive environment resistant NK cells
  • Showcasing preliminary efficacy against virus infected targets and hep G2 cell lines
  • Discussing pathways of NK dysregulation in hepatitis B infections

4:30 pm Close of Conference Day Two